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Abstract
A flow-injection system with biamperometric detection for L-ascorbic acid (vitamin C) determination was designed. The method is based on L-ascorbic acid oxidation to dehydroascorbic acid, in acidic medium, using iodine-iodide solution as oxidizing reagent. The iodine amount consumed in the redox reaction was biaperometrically detected and it was proportional to the amount of L-ascorbic acid from the sample. The calibration graph was linear over the range of concentration 5×10−5-5×10−4 M of vitamin C. The RSD for a 2.5×10−4 M standard solution of vitamin C was 1.08% (n=10) and the throughput was 60 samples h−1. The selectivity of the proposed FIA method was verified using an ascorbate oxidase solution as carrier stream. It was found that L-ascorbic acid could be determined in natural products with a good selectivity and rapidity.