Simultaneous Determination of Two - Component Mixtures in Pharmaceutical Formulations Containing Chlordiazepoxide by Ratio Spectra Derivative Spectrophotometry

ABSTRACT

The ratio spectra derivative spectrophotometry was applied for the simultaneous determination of chlordiazepoxide (CH) with clidinium bromide (CL), pipenzolate bromide (PI) or amitriptyline hydrochloride (A) in binary mixtures. The analytical signals were measured at 243.1 and 214.8 nm in the CH-CL mixture, 271.4 and 256.7 nm in the CH - PI mixture, and 264.9 and 218.0 nm in the CH-A mixture for CH and its co-existing compounds, respectively, in the first derivative mode in methanol. Calibration graphs were established for 2.0 - 38.0 μml⁻¹ CH and 4.0 - 20.0 μgml⁻ CL in CH - CL mixture, for 2.0 - 38.0 μgml⁻¹ CH and 4.0 - 32.0 μgml⁻¹ PI in CH - PI mixture, and for 2.0 - 28.0 μgml⁻¹ CH and 4.0-36.0 μgml⁻¹ A in CH - A mixture. The method was successfully applied to the pharmaceutical formulations containing the above-mentioned drug combinations without any interference by the excipients. Vierordt's method was also developed for a comparison method.

Keywords::

• Chlordiazepoxide
• clidinium bromide
• pipenzolate bromide
• amitriptyline hydrochloride
• ratio spectra derivative spectrophotometry
• Vierordt's method