Comparison of Micellar Electrokinetic Chromatography with HPLC for the Separation of Hematoporphyrin Derivatives

ABSTRACT

A mixed SDS micelle and BSA buffer system was used in the micellar electrokinetic chromatography (MEKC) separation of hematoporphyrin derivatives (HPD) at pH 8.0 with untreated capillaries. The effects of altering the composition of sodium dodecyl sulfate (SDS) electrolyte solution on the separation efficiency of the hematoporphyrin derivatives were presented. The results show that separation efficiencies were enhanced by using a mixture of SDS and BSA. The results demonstrated that CE methodology can compete with well-established techniques such as HPLC for the separation of biomedical and pharmaceutical samples with regard to time and expense of analysis.

Keywords::

• Hematoporphyrin derivatives
• Micellar electrokinetic chromatography (MEKC)
• Capillary electrophoresis (CE)
• Bovine serum albumin (BSA)
• High-performance liquid chromatography (HPLC)