Chemiluminescence Determination of Gluconic Acid in Pharmaceutical Formulations using Ru(bipy)₃²⁺ – KIO₄ – Ce(IV) System

ABSTRACT

A new chemiluminescence method for determination of gluconic acid is described. The method is based on the chemiluminescence reaction of gluconic acid with KlO₄, Ru(bipy)₃ ²⁺ (bipy=2,2'-pyridyl) and Ce(IV) in sulfuric acid medium. Under the optimum conditions, the concentration of gluconic acid is proportional to the CL intensity in the range of 7.6x10⁻⁸ ˜1.5x10⁻⁵ mol/L. The limit of detection is 1.5x10⁻⁸ mol/L and the relative standard deviation is 3.5% for 5.0x10⁻⁷ mol/L gluconic solution (n=11). The method is relatively free from interferences from common excipients and co-existing drugs and has been successfully applied to the analysis of gluconic acid in pharmaceutical formulations.

Keywords::

• Chemiluminescence
• Gluconic acid
• Pharmaceutical analysis
• Tris-(2,2'-bipyridyl)ruthenium(II)
• Cerium(IV)
• Periodate