Carbon Disulfide Metabolites Excreted in the Urine of Exposed Workers

Abstract

The authors have isolated and identified in the urine of exposed workers the metabolites of carbon disulfide which are causing positive iodineazide reaction (IAR). By thin-layer chromatography (TLC), it was established that three metabolites are present in the urine giving positive IAR. It was postulated that 2-mercapto-2-thiazolinone-5 could be one of them. For comparison, this compound was accordingly synthesized. After concentration of the urine in a vacuum, chromatography of an aluminum oxide column, elution with a chloroformmethanol mixture, and TLC, 2-mercapto-2-thiazolinone-5 was isolated. Identification of the isolated compound was performed by TLC, gas chromatography, infrared spectrophotometry, elemental microanalysis, and mass spectrometry using the synthesized product as the reference substance.