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Abstract
The effects of incubation time and deficiencies of calcium and nitrate on zoospore production were investigated in Botrydiopsis arhiza Borzi, B. intercedens Vischer et Pascher, Bumilleriopsis peterseniana Vischer, Pseudobumilleriopsis pyrenoidosa Deason et Bold, and Ophiocytium maius Naegeli. Release of zoospores occurred after the transfer of stationary phase, vegetative cells into fresh Bold's basal medium. Maximum yields of zoospores were produced on the second day after transfer, 0·5–1·0 h after the start of the light period of a 12–12 h light-dark cycle. Lack of calcium or nitrate reduced the yield of zoospores in each taxon. Only O. maius produced large numbers of motile cells in nitrate-free medium.