Functional interactions of manno-oligosaccharides with dietary threonine in chicken gastrointestinal tract. II. Mucosal development, mucin dynamics and nutrient utilisation

Abstract

1. A 3 × 2 factorial experimental design was used to investigate the interaction between threonine concentration (0·7, 1·0 and 1·3 of National Research Council (NRC), 1994, recommendations) and manno-oligosaccharides (MOS) supplemented at 0 and 2 g/kg on growth performance in relation to intestinal flow of crude mucins, mucosal development and nutrient utilisation.

2. There was no interaction between MOS and dietary threonine in any performance variable analysed, except for body weight gain during the period to 14 d of age, where body weight gain was significantly lower in birds fed excess threonine in the absence of MOS. Dietary MOS was also observed to significantly increase the body weight gain at deficient and adequate concentrations of threonine.

3. Dietary treatments had no significant effect on either the ileal external muscularis thickness or crypt depth. However, there was a MOS and threonine interaction in the ileal villus to crypt ratio and ileal crude mucin output with both being increased only at the adequate threonine concentration.

4. Dietary MOS tended to interact with threonine to increase the ileal uptake of D-glucose and L-threonine, but the effect was only apparent in birds fed on the deficient or excess threonine diet. There was no significant interaction between MOS and threonine on ileal digestibility of amino acids. Supplementation of MOS or increased dietary threonine significantly increased the apparent and standardised ileal digestibility of threonine.

5. Results from the current study indicate the possible link between the modulating effects of these supplements on intestinal mucosal development and mucin dynamics. This, in turn, may suggest a relatively higher proportion of mature enterocytes and absorptive area, which would be expected to improve the capacity for digestion and mucosal nutrient absorption.

Acknowledgements

This study was supported by Alltech Biotechnology (Nicholasville, KY, USA). The authors are grateful for the financial support in the form of International Postgraduate Research Scholarship from the Australian Government and the University of New England, Armidale, NSW, Australia.