Chicken dendritic cells and type II pneumocytes express a common intracellular epitope

Abstract

1. CVI-ChNL 74·3, a dendritic cell-specific monoclonal antibody (mAb) also identifies chicken lung granular pneumocytes (type II pneumocytes), which produce surfactant.

2. The 74·3 mAb does not cross-react with any other avian or mammalian granular pneumocyte, and provides a convenient tool for monitoring the status of type II pneumocytes in the chicken lung.

Acknowledgements

The anti-SP-A and SP-B were a generous gift from Professor Whitsett (Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA). The anti-laminin monoclonal antibody (clone 31) was obtained from Developmental Studies Hybridoma Bank, Iowa City, IA 52242, USA. The authors are grateful to Jutka Fügedi and Zsuzsa Vidra for technical assistance. The work was supported by OTKA grant number 69061.