Advanced search
Publication Cover
British Poultry Science Volume 54, 2013 - Issue 2
Submit an article Journal homepage
167
Views
1
CrossRef citations to date
0
Altmetric
Physiology, Endocrinology & Reproduction

In vitro initiation of the acrosome reaction in the emu (Dromaius novaehollandiae)

R.P. Singh School of Animal Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia , Crawley, WA, 6009, Australia; Institute of Agriculture, The University of Western Australia , Crawley, WA, 6009, Australia; Physiology and Reproduction Division, Central Avian Research Institute , Bareilly, India; Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History , Coimbatore, India
,
P.K. Rybnik-Trzaskowska School of Animal Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia , Crawley, WA, 6009, Australia; Institute of Agriculture, The University of Western Australia , Crawley, WA, 6009, Australia; Department of Genetics and Animal Breeding, Warsaw University of Life Sciences , Warsaw, 02-786, Poland
,
U. Farooq School of Animal Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia , Crawley, WA, 6009, Australia; Institute of Agriculture, The University of Western Australia , Crawley, WA, 6009, Australia
,
I.A. Malecki School of Animal Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia , Crawley, WA, 6009, Australia; Institute of Agriculture, The University of Western Australia , Crawley, WA, 6009, Australia; Department of Animal Sciences, University of Stellenbosch , Matieland, South Africa
,
K.V.H. Sastry School of Animal Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia , Crawley, WA, 6009, Australia; Institute of Agriculture, The University of Western Australia , Crawley, WA, 6009, Australia; Physiology and Reproduction Division, Central Avian Research Institute , Bareilly, India
&
G.B. Martin School of Animal Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia , Crawley, WA, 6009, Australia; Institute of Agriculture, The University of Western Australia , Crawley, WA, 6009, Australia
Pages 259-264 | Published online: 07 May 2013
 
Sample our Food Science & Technology journals, sign in here to start your access, latest two full volumes FREE to you for 14 days

Abstract

1. An assessment of the efficiency of the acrosome reaction (AR) provides an important predictor of the fertilizing potential of semen and for diagnosis of the causes of infertility. A standardized protocol was therefore developed for initiation of the acrosome reaction in emu spermatozoa in vitro, and the role of CaCl2 or perivitelline membrane (PVM) proteins in determining the outcome of the reaction was investigated.

2. The acrosome reaction (assessed by FITC-PNA) was successfully induced in live spermatozoa by incubation for 2 min in NaCl-TES medium supplemented with 5 mM CaCl2. The maximum response was 32% live acrosome-reacted spermatozoa (LAR) achieved after 10 min incubation.

3. Compared to the outcome with 5 mM CaCl2 or PVM protein alone, the response was significantly better with a combination of PVM protein and CaCl2.

4. A significant variation in the percentage of LAR spermatozoa among individual males was observed. No treatment affected the percentage of dead acrosome-reacted spermatozoa.

5. The results emphasize the important role played by both PVM proteins and Ca2+ in the in vitro initiation of the acrosome reaction.

ACKNOWLEDGEMENTS

The study was supported by Department of Education, Employment and Workplace Relations, Government of Australia, through Endeavour Research Awards to both Dr R.P. Singh and Dr P.K. Rybnik-Trzaskowska and by the Rural Industries Research and Development Corporation (Australia).

Log in via your institution

Access through your institution

Log in to Taylor & Francis Online

Restore content access

Restore content access for purchases made as guest
Purchase options * Save for later

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 169.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.