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Abstract
1. The low-density lipoprotein receptor-related protein 8 (LRP8), a member of the low-density lipoprotein receptor (LDLR) gene family, participates in the supplying of lipid during follicular development. The objective of the study was to identify and characterise the LRP8 gene in goose.
2. A 2867 bp fragment that covered the complete coding region (CDS) of goose (Anser cygnoides) LRP8 gene was cloned. It encoded a protein of 917 amino acid residues containing a 24-amino acid signal peptide and 5 functional domains. The goose LRP8 showed high nucleic acid and amino acid identities with those in other species.
3. Similarly to duck LRP8 gene, two splice variants of LRP8, LRP8-1 (containing 8 ligand-binding repeats) and LRP8-2 (containing 7 ligand-binding repeats), were identified in goose.
4. Semi-quantitative RT-PCR analysis indicates that the LRP8-1 transcript is expressed in heart, liver, spleen, lung, kidney, breast muscle, duodenum, hypothalamus, pituitary and ovary, negligible or absent in sebum and oviduct, and the LRP8-2 transcript is widely expressed in all examined tissues.
5. A total of 7 SNPs were identified in the coding region of the goose LRP8 gene.
ACKNOWLEDGEMENTS
The authors thank Prof. Yanzhang Gong for the kind advice on this research (Huazhong Agricultural University). This study was supported by the Fund of China Agriculture Research System (grant no. CARS-43), the Key Research Program of Prospering Agriculture Through Science and Technology of Shanghai Management Office (grant no. 2009: 2-1) and the Construction Program of Zhedong White Goose Breeding Platform.
SUPPLEMENTARY INFORMATION
Supplementary Figures 1 and 2 can be accessed via the online version of this article at: http://dx.doi.org/10.1080/71668.2014.904501