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Abstract
1. The aim of this study was to develop a suitable method for the analysis of florfenicol (FF) and its metabolite florfenicol amine (FFA) in chicken eggs and to determine FF and FFA residue depletion in eggs of laying hens.
2. The analytes were extracted from yolk, albumen and whole egg by phosphate buffer and ethyl acetate. Following purification, samples were analysed by high-performance liquid chromatography.
3. Fifty laying hens were divided into 5 groups, and each hen received doses of 20 mg/kg FF: Group 1 (received a single oral dose by gavage); Group 2 (a single intramuscular dose); Group 3 (a single subcutaneous dose); Group 4 (multiple oral doses for 3 d) and Group 5 (multiple oral doses for 5 d).
4. Limits of detection and of quantitation values were 1.94 and 6.45 g/109 g (ppb) for FF, respectively, and 0.48 and 1.58 ppb for FFA, respectively. Relative standard deviation values of intra-day and inter-day variation below 11% also confirmed the usefulness of the method for analysing FF and FFA in eggs.
5. From the first day of both oral and parenteral administration, FF and FFA were detected at 0.1% and 0.08% of dosage, respectively, and 57% of the drugs were eliminated from the egg yolk. Elimination time of FF was 8 d in Groups 1, 2 and 3; 9 d in Group 4 and 10 d in Group 5.
ACKNOWLEDGEMENTS
This study was supported by Ankara University Scientific Research Projects (10B3338003). The authors sincerely thank Ankara University Scientific Research Committee. This article was originally an oral presentation at the 12th International Congress of the European Association for Veterinary Pharmacology and Toxicology, 8–12 July 2012, Noordwijkerhout, the Netherlands. It was published as a conference abstract in a Special Issue of Journal of Veterinary Pharmacology and Therapeutics (35(3): 30). The abstract has been modified and extended into a full research article for the current publication. The authors declare no competing financial interest.