Effect of extracted galactoglucomannan oligosaccharides from pine wood (Pinus brutia) on Salmonella typhimurium colonisation, growth performance and intestinal morphology in broiler chicks

Abstract

1. An in vitro and in vivo study was conducted to evaluate the fermentability of isolated galactoglucomannan oligosaccharides (GGMs) and the influence of their feeding on shedding and colonisation of Salmonella typhimurium, growth performance and intestinal morphology in broiler chicks.

2. The in vitro data demonstrated that three probiotic lactic acid bacteria namely Lactobacillus casei, L. plantarum and Enterococcus faecium were able to ferment the extracted oligosaccharides and other tested sugars on a basal de Man Rogosa Sharpe media free from carbohydrate.

3. For the in vivo experiment, 144 one-d-old male Ross 308 broiler chicks were divided into 6 experimental treatments (with 4 replicates) including two positive and negative controls which received a basal maize-soybean diet without any additives, supplementation of three levels of isolated GGMs (0.1%, 0.2% and 0.3%) and a commercial mannanoligosaccharide (MOS) at 0.2% to the basal diet. All birds except those in the negative control group were challenged orally with 1 × 10⁸ cfu of S. typhimurium at 3-d post-hatch.

4. The results revealed that challenge with S. typhimurium resulted in a significant reduction in body weight gain, feed intake, villus height, villus height to crypt depth ratio and villus surface area in all of infected chicks. Birds that were given GGMs or MOS showed better growth performance, increased villus height and villus surface area and decreased S. typhimurium colonisation than the positive control birds. GGM at 0.2% level was more effective than the other treatments in improving growth rate as well as gut health of broiler chicks.

ACKNOWLEDGEMENTS

The authors greatly acknowledge the support received from the staff of the wood and paper laboratory, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

DISCLOSURE STATEMENT

No potential conflict of interest was reported by the authors.