Physiological responses to central and peripheral injection of polyinosinic-polycytidylic acid in chicks

ABSTRACT

1. The purpose of the present study was to determine if intracerebroventricular (ICV) and intraperitoneal (IP) injection of polyinosinic-polycytidylic acid (poly I:C), a viral mimetic that binds to toll-like receptor-3 (TLR3), affects food intake, voluntary activity, cloacal temperature, plasma corticosterone (CORT) and glucose concentrations, and crop emptying rate in chicks (Gallus gallus).

2. Both ICV and IP injection of poly I:C significantly decreased food intake.

3. IP but not ICV injection of poly I:C significantly suppressed voluntary activity, whereas ICV injection decreased time spent sitting. Both ICV and IP injection of poly I:C significantly increased plasma CORT and glucose concentration. Neither ICV nor IP injection of poly I:C significantly affected cloacal temperature.

4. In addition, ICV injection of poly I:C significantly reduced crop emptying rate, whereas IP injection had no effect.

5. These results suggested that central TLR3 is related to anorexia, stress response and retardation of crop emptying while peripheral TLR3 is related to anorexia, change in behaviour and stress responses during viral infection in chicks.

KEYWORDS:

• Chick
• cloacal temperature
• corticosterone
• crop emptying rate
• feeding
• polyinosinic-polycytidylic acid
• voluntary activity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI [Grant 25450398 and 16K07991].