Estrogen enhances the expression of a growth-associated long noncoding RNA in chicken liver via ERα

ABSTRACT

1. The long noncoding RNA lncGLM is significantly differentially expressed in the livers of peak-laying hens compared with that in the livers of pre-laying hens, but its potential biological role and expression regulation are unclear.

2. To explore the potential biological function of lncGLM, single nucleotide polymorphism (SNP) detection and association analysis were carried out in the Gushi×Anka F₂ resource population.

3. The tissues and spatiotemporal expression characteristics of lncGLM were analysed by real-time quantitative PCR. The effects of 17β-oestradiol on the expression of lncGLM expression were analysed through in vitro and in vivo experiments.

4. The results showed that a g.19069338 T > C SNP was present in lncGLM. Association analysis revealed that lncGLM was significantly associated with body slanting length at 12 weeks, body weight at 12 weeks, shank length at four weeks, chest depth at eight weeks, pelvic width at 12 weeks, eviscerated weight, head weight, pancreas weight, pectoralis weight, leg muscle weight, muscular stomach weight rate, pancreas weight rate, carcase weight, aspartate aminotransferase, creatinine and pectoral muscle water loss rate.

5. The expression of lncGLM in the liver was higher than that in other sampled tissues. In addition, the expression of lncGLM in the liver was significantly higher in the peak-laying period than at the pre-laying period. Both in vitro and in vivo experiments showed that lncGLM expression was regulated by 17β-oestradiol via oestrogen receptor alpha (ER-α). These results demonstrated that the chicken lncGLM gene is highly expressed in liver tissue and regulated by oestrogen through ER-α.

KEYWORDS:

• lncGLM
• SNP
• growth
• oestrogen
• expression regulation

Disclosure statement

The authors declare that they have no competing interests.

Ethics statement

All animal experiments were conducted in accordance with the protocol approved by the Animal Care Committee of Henan Agricultural University (Zhengzhou, People’s Republic of China) approved this study (approval number 11–0085).

Additional information

Funding

This research was supported by the National Natural Science Foundation of China [(No. 31902146, and No. U1704233)]; the China Agriculture Research System [(No. CARS-40-K04)]; the China postdoctoral science foundation [(2017M620301)].