Characteristics of antioxidant substances and identification of antioxidant peptides in duck embryo eggs

ABSTRACT

1. Oxidative stress in duck embryos undergoes dynamic change during incubation. However, the detailed change characteristics has not been studied yet. Here, we explored the dynamic change characteristics of different antioxidant substances in duck embryo eggs during incubation.

2. The following trial assayed antioxidant substances, including vitamins E (VE) and C (VC), glutathione (GSH), reduced glutathione (reduced GSH), oxidative glutathione (GSSG), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-px), and malonic dialdehyde (MDA). Five antioxidant peptides (DY1, DY2, DY3, DY4 and DY5) were screened and their protective effect on HepG2 cells and their mechanism of action determined.

3. The results showed that antioxidant substances underwent a dynamic change during incubation through various mechanisms. Moreover, the T-AOC of duck embryos at different incubation times, firstly increased and then decreased, reaching the highest level on d 15 during incubation. The peptide DY2 (TVDGPSGKLWRD) exhibited high antioxidant activity in vitro, and is known to regulate the apoptosis pathway in mitochondria.

4. The data indicated that duck embryos can be used for the development of related antioxidant products and purification of new bioactive peptides.

KEYWORDS:

• Duck embryo
• antioxidant
• incubation
• antioxidant peptide

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Chongqing Scientific Research Institution Performance incentive project (No. 19539), Agriculture development fund of Chongqing (No. 18508), Special funds for performance guidance of scientific research institutions (No. cstc2018jxj10048), the National Natural Science Foundation of China No. 31571782 and 31771975, and the Natural Science Foundation of Chongqing CSTC (No. cstc2018jcyjAX0765)