PLGA nanoparticle with Amomum longiligulare polysaccharide 1 increased the immunogenicity of infectious bursal disease virus VP2 protein

ABSTRACT

1. The purpose of this study was to create ALP1-VP2-PLGA nanoparticle (AVPN) and to study the immunogenicity of AVPN. AVPN was prepared and observed by scanning and transmission electron microscopies.

2. Chickens were divided into five groups and vaccinated with normal saline, VP2 protein, ALP1 and VP2 protein, AVPN or PLGA, respectively. After 28 days, the immune organ indexes were calculated; specific antibody levels in blood were detected by enzyme-linked immunosorbent assay (ELISA). Additionally, the spleen and bursa of Fabricius were determined by HE staining, immunological cytokine mRNA levels in bursa of Fabricius were detected by qPCR andchicken body weight was determined.

3. The results indicated that AVPN was a spherical nanoparticle with a diameter of about 85 nm. It increased bursal indexes and IBDV-specific antibody levels and promoted the expression of IL-2 mRNA in blood and TNF-α and IgG mRNA in bursa of Fabricius. This promoted growth.

4. This study suggested that AVPN can increase immunogenicity of VP2 protein, and it could possibly be used as an IBDV subunit vaccine.

KEYWORDS:

• Infectious bursal disease
• VP2
• Amomum longiligulare polysaccharide 1
• poly-lactic-co-glycolic acid
• adjuvant

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This project was supported by Hainan Province Science and Technology Special Fund (Grant No. ZDYF2022XDNY141), National Natural Science Foundation of China (Grant No. 32160808) and Scientific Research Staring Foundation of Hainan University (Grant No. KYQD(ZR)1825).