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Caryologia
International Journal of Cytology, Cytosystematics and Cytogenetics
Volume 31, 1978 - Issue 4
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Original Articles

Production of Trisomics in Triticum Durum Desf. by the Conversion Technique

Ottenimento di trisomici in Triticum durum Desf. mediante la tecnica di conversione

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Pages 487-491 | Received 01 Jul 1978, Published online: 30 Jan 2014
 

SUMMARY

Four nulli-tetrasomic lines of Triticum aestivum var. Chinese Spring were backcrossed to T. durum var. Cappelli.

The plants of the BC1 generation with chromosome number 2 n = 29 (ascertained by mitotic analysis of root tips) were analyzed in metaphase I in order to distinguish the real trisomic plant from the monosomic addition line due to the presence of one chromosome of the D genome. At least one trisomic plant in each BC, family was isolated.

The conversion technique gives the possibility of obtaining the wanted trisomic lines, while other methods need a particular and laborious program in order to identify the extra chromosome.

RIASSUNTO

Quattro linee nulli-tetrasomiche di T. aestivum var. Chinese Spring sono state reincrociate con il T. durum var. Cappelli.

Le piante della generazione BCi con numero crosomico 2 n = 29 (accertato mediante analisi mitotica degli apici radicali) sono state analizzate in metafase I della meiosi allo scopo di distinguere le piante trisomiche per un cromosoma del genoma A o B (presentanti 13 bivalenti e 1 trivalente) dalle piante con un cromosoma del genoma D (14 bivalenti e 1 univalente). Per ciascuna progenie è stato possibile isolare almeno una pianta trisomica.

La tecnica di conversione permette di ottenere linee trisomiche per un determinato cromosoma, evitando quindi l'identificazione che di solito richiede un programma specifico e laborioso.

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