Abstract
The genus Artemia is an interesting model organism for evolutionary studies since many factors driving speciation have been observed in the genus. It has been suggested that in this genus heterochromatin content could be a reliable criteria to establish taxonomic identity of populations. In the present work the heterochromatin content was determined in 4 populations of A. franciscana and seven populations of A. persimilis, six from Argentina and one from Chile. Heterochromatin was revealed by C-banding and the heterochromatin content (measured as chromocenter number and C-positive area in nauplii interphase nuclei) was compared. A standardized procedure to evaluate the heterochromatin content is here proposed so that the results from different laboratories could be compared. We analyze the intra and interspecific variation in heterochromatin content in the different Artemia populations and we discuss the value of this trait as a population and/or specific cytogenetic marker.