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Caryologia
International Journal of Cytology, Cytosystematics and Cytogenetics
Volume 63, 2010 - Issue 3
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Original Articles

Genetic Characterisation in two species of Catopsilia (Pieridae: Lepidoptera) by RAPD-PCR technique

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Pages 250-256 | Received 07 Sep 2009, Accepted 09 Jun 2010, Published online: 10 Feb 2014
 

Abstract

Present study was carried out to characterize the two species of butterfl ies i.e. Catopsilia crocale and Catopsilia pyranthe at molecular level by using three random primers. The banding patterns obtained and the dendrograms drawn on the basis of presence and absence of bands revealed that P2 is most useful in characterizing the two species, since it shows difference between the two sexes of each species and at the same time also clusters them together as separate species. P1 showed least similarity within the individuals of the same species. Though P3 showed relationships between male and female individuals of C. pyranthe and also clusters them together as a species, but it placed the females of C. crocale far from males of this species. Therefore, P2 is recommended for further study.

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