Soil Microbial Community Composition and Diversity in the Rhizosphere of a Chinese Medicinal Plant

Abstract

Rehmannia glutinosa is an important medicinal plant, but there is a serious problem of decreasing productivity with its continuous cropping on the same land. We hypothesize some relationships between this problem and the disturbed soil ecosystem. In this work, two community‐based microbiological measurements, community‐level physiological profiling (CLPP) using Biolog sole carbon (C) source utilization tests and phospholipid ester–linked fatty acid (PLFA) profiles, were used to evaluate soil microbial community function and composition of different R. glutinosa cropping soils. Field investigation showed that the problems with continuous cropping occurred not only in 2‐year continuous fields but also in 5‐year rotation fields. Soil basal respiration and metabolic quotient were significantly greater in R. glutinosa cropping soils than in the noncropping controls. In contrast, the Shannon index from the Biolog data set was lower in R. glutinosa cropping soils. Both CLPP‐ and PLFA‐based principal component analyses (PCA) showed distinct groupings of soil microbial communities in R. glutinosa rhizosphere, and 11 PLFAs representing different microbes were identified from the principal component scores of PLFAs. Among these, an abundance of PLFA 18:2ω6,9, which is a biomarker of soil fungi, was significantly higher in R. glutinosa cropping soils than control soils. These results suggest an alteration of soil microbial community following R. glutinosa cropping, and this might be an important reason for the constraints associated with continuous cropping.

Keywords:

• CLPP
• continuous cropping
• PLFA
• Rehmannia glutinosa
• soil microbial community

ACKNOWLEDGMENTS

We thank Mr. Wang Tian Liang, vice president of the Institute of Agricultural Sciences of Wen Xian County in Henan Province, for his kind help in site selection and field management. This work was funded by grants from the China National Natural Science Foundation (30472155, 30871600) and Beijing Natural Science Foundation (5062035).