Potential of Chemically Labile Fractions to Measure Mineralizable Soil Nitrogen

Abstract

Nitrogen (N) in the soil is largely organic and is available to crops only after it is mineralized to inorganic N by microbial or enzyme action. To develop a soil test for guiding N applications, a method to predict the relative amount of organic N that will mineralize in a growing season is necessary. Several chemical analysis methods proposed in the literature to measure mineralizable N were examined for chemical interference, measurement precision, response to procedure modifications, and ability to distinguish differences among soils. The chemical analyses examined involved various acid or alkaline hydrolysis, with the resulting inorganic ammonium N measured by steam distillation and manual or automated diffusion. A gelatinous precipitate in the filtered and neutralized 6 M hydrochloric acid (HCl) hydrolysis solution interfered with magnesium oxide (MgO) diffusion traditionally used to measure inorganic ammonium N. Removing the precipitate appeared to circumvent the interference. The precipitate did not appear to interfere with the sodium hydroxide (NaOH) diffusion. The 6 M HCl hydrolysis extracted 34 to 103% of clay‐fixed ammonium in the soils. Steam distillation was shown to be an acceptable alternative to diffusion for measuring NaOH‐labile N. The vigor of NaOH measurement conditions caused differences in results, showing that precise and reproducible conditions are necessary. Several methods were closely correlated (r² > 0.62) with N mineralized during aerobic incubations and could be considered for further evaluation for soil N testing. This study showed that modifications are required to several proposed analytical methods to improve their potential to estimate mineralizable N for fetilizer or other amendment recommendations for crop production

Keywords:

• Acid hydrolysis
• alkali treatment
• mineralization
• nitrogen
• soil testing