Preparation of diazoresin/poly(ethylene oxide)_x-b-polystyrene-b-poly(ethylene oxide)_x capillary coating for analysis of proteins by capillary electrophoresis

ABSTRACT

Considering the conventional silane coupling agents in the surface modification of the application of toxicity, moisture sensitive and other defects. We proposed a new method of preparing an environmentally friendly capillary coating by using a photosensitive diazoresin (DR) and poly(ethylene oxide)_x-b-polystyrene-b-poly(ethylene oxide)_x (PEO_x-b-PS-b-PEO_x, x = 1, 2, 3) was coated on the inner surface of the capillary, and the separation performance of the PEO_x-b-PS-b-PEO_x capillary coating was investigated thoroughly. The coating shields the adsorption of protein on the inner wall of the capillary and baseline separation of the four proteins of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be realized within 20 minutes.

KEYWORDS:

• Capillary electrophoresis
• diazoresin
• poly(ethylene oxide)_x-b-polystyrene-b-poly(ethylene oxide)_x
• protein separation

Additional information

Funding

This work is financially supported by the National Natural Science Foundation of China (21574072, 21675091), the Taishan Young Scholar Program of Shandong Province (tsqn20161027), the Key Research and Development Project of Shandong Province (2016GGX102028, 2016GGX102039, 2017GGX20111), the Project of Shandong Province Higher Educational Science and Technology Program (J15LC20), the People's Livelihood Science and Technology Project of Qingdao (166257nsh, 173378nsh), the Innovation Leader Project of Qingdao (168325zhc), the Open Research Project of State Key Laboratory of Molecular Engineering of Polymers of Fudan University (K2016-01), and the First Class Discipline Project of Shandong Province.