Preparation of hydroxypropyl cellulose-poly (2-Methacryloyloxyethyl phosphorylcholine) coating for the analysis of proteins by capillary electrophoresis

Abstract

We reported a new method for preparing coated capillaries in this paper. First, we combined hydroxypropyl cellulose (HPC) and 2-Methacryloyloxyethyl phosphorylcholine (MPC) to form hydroxypropyl cellulose-poly (2-Methacryloyloxyethyl phosphorylcholine) (HPC-PMPC) by atom transfer radical polymerization (ATRP). Then the photosensitive polymer diazoresin (DR) was used as a linker to the inner wall of the capillary, and finally exposed to ultraviolet light to form a stable covalent bond coating. Protein adsorption on the inner surface of capillary was effectively inhibited. The cytochrome c (Cyt-c), lysozyme (Lys), ribonuclease A (RNase A) and bovine serum albumin (BSA) were separated obviously using capillary electrophoresis (CE) within 15 minutes and showed good stability and repeatability.

Keywords:

• Capillary electrophoresis
• diazoresin
• coating
• hydroxypropyl cellulose
• protein separation

Additional information

Funding

This study was funded by the National Natural Science Foundation of China (21675091, 21574072, 51703105, 21874078), the Taishan Young Scholar Program of Shandong Province (tsqn20161027), the Major Science and Technology Innovation Project of Shandong Province (2018CXGC1407), the Key Research and Development Project of Shandong Province (2016GGX102028, 2016GGX102039, 2017GGX20111), the Natural Science Foundation of Shandong Province (ZR2017BEM012), the Project of Shandong Province Higher Educational Science and Technology Program (J15LC20), the People's Livelihood Science and Technology Project of Qingdao (166257nsh, 173378nsh), the Innovation Leader Project of Qingdao (168325zhc), the China Postdoctoral Science Foundation (2018M630752), and the First Class Discipline Project of Shandong Province.