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Abstract
The embryonic striatal temperature sensitive immortalized ST14A-cell line was characterized in vitro by immunocytochemistry when cultured at 33°C and at nonpermissive temperature of 39°C for up to 14 days. At 33°C in DMEM/10% FCS, cells proliferated, were extensively expressing the neural progenitor cell markers nestin and vimentin contrary to neuronal markers. However, when cultured at 39°C the proliferation was delayed and cells began to increase the expression of neuronal markers, followed by a decrease of nestin and vimentin. In serum-free medium the process of neuronal differentiation became more obvious, indicating the potential to use these cells for experimental restorative therapies.
| Abbreviations | ||
| BSA | = | bovine serum albumin |
| CNS | = | central nervous system |
| DMEM | = | Dulbecco's modified Eagle's medium |
| FCS | = | fetal calf serum |
| MAP2 | = | microtubule associated protein 2 |
| MAP5 | = | microtubule associated protein 5 |
| NF160 | = | neurofilament 160 |
| NF200 | = | neurofilament 200 |
| NeuN | = | neuron-specific nuclear protein |
| Abbreviations | ||
| BSA | = | bovine serum albumin |
| CNS | = | central nervous system |
| DMEM | = | Dulbecco's modified Eagle's medium |
| FCS | = | fetal calf serum |
| MAP2 | = | microtubule associated protein 2 |
| MAP5 | = | microtubule associated protein 5 |
| NF160 | = | neurofilament 160 |
| NF200 | = | neurofilament 200 |
| NeuN | = | neuron-specific nuclear protein |