ABSTRACT
Purpose: The current study aims at investigating the downstream targets of spinal Annexin A10 in modulating neuropathic pain.
Materials and methods: Paw withdrawal latency and paw withdrawal threshold were measured to evaluate the pain-associated behaviour in rats. The expression of spinal Annexin A10, phosphorylated-extracellular regulated kinase 1/2 and extracellular regulated kinase were detected by western blotting. The level of tumour necrosis factor-α and interleukine-1β was tested by enzyme-linked immunosorbent assay (ELISA) kits.
Results: Chronic constrictive injury caused pain hypersensitivity in rats, along with increased expression of spinal Annexin A10, phosphorylated-extracellular regulated kinase 1/2, tumour necrosis factor-α and interleukine-1β in rats. Knockdown of spinal Annexin A10 suppressed the chronic constrictive injury-induced hyperalgesia, and inhibited the chronic constrictive injury-induced increased expression of phosphorylated-extracellular regulated kinase 1/2, tumour necrosis factor-α and interleukine-1β in the spinal cord. Inhibition of spinal extracellular regulated kinase activation decreased the release of tumour necrosis factor-α and interleukine-1β, but did not change the increased expression of Annexin A10 caused by chronic constrictive injury.
Conclusions: Annexin A10 contributed to the development of neuropathic pain by activating spinal extracellular regulated kinase signalling and the subsequent release of tumour necrosis factor-α and interleukine-1β in the spinal cord.
KEYWORDS:
Acknowledgments
The authors gratefully thank Li-Hong Sun from Zhejiang University for her help in cheking the English writing of this study.
Disclosure of interest
The authors report no conflicts of interest.
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Notes on contributors
Feng Li
Feng Li is a PhD candidate at The First People's Hospital of Yancheng, Nantong University. He majored in the research of the pharmacology of anesthesia and pain.
Zhou-Ya Xue
Zhou-Ya Xue is a MM at The First People's Hospital of Yancheng, Nantong University. She was interested in the research of the mechanism of postoperative pain and postoperative cognitive dysfunction.
Xiang Liu
Xiang Liu is a MM at The First People's Hospital of Yancheng, Nantong University. He majored in clinical pain treatment and was dedicated to exploring innovative and effective treatment for pain.
Gang Bai
Gang Bai is a MM at The First People's Hospital of Yancheng, Nantong University. He majored in the research of the peripheral and central mechanisms of visceral pain, neuropathic pain, and inflammatory pain.
Yuan-Lin Wang
Yuan-LIn Wang is a PhD candidate at The First People's Hospital of Huai'an, Nanjing Medical University. She devoted to doing the research of the molecular biology mechanism of neuropathic pain and exploiting new therapeutic opportunities for neuropathic pain.