Neuritin provides neuroprotection against experimental traumatic brain injury in rats

ABSTRACT

Objectives: Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. Neuritin is a neurotrophic factor that regulates neural growth and development. However, the role of neuritin in alleviating TBI has not been investigated.

Methods: In this study, Sprague Dawley rats (n = 144) weighing 300 ± 50 g were categorized into control, sham, TBI and TBI + neuritin groups. The neurological scores and the ultrastructure of cortical neurons, apoptotic cells and caspase-3 were measured by using Garcia scoring system, transmission electron microscopy, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, Western blot analysis and real-time RT-PCR at various time points post-TBI.

Conclusions: Our findings indicated that neuritin plays a protective role in TBI by improving neurological scores, repairing injured neurons and protecting the cortical neurons against apoptosis through inhibition of caspase-3 expression. Further investigation of the molecular mechanisms underlying caspase-3 inhibition by neuritin will provide a research avenue for potential TBI therapeutics.

KEYWORDS:

• Traumatic brain injury
• neuritin
• apoptosis
• caspase-3
• neuroprotection

Disclosure statement

No competing financial interests exist.

Additional information

Funding

This study was supported by grants from National Natural Science Foundation of China [grant number 81360185]; Research and Innovation Project of Graduate Students in Xinjiang Uygur Autonomous Region [grant number XJGRI2017041]; International Cooperation Project of Shihezi University [grant number GJHZ201704].