Comparative proteomic profile in bee- and manually-collected Taraxacum officinale pollen

Abstract

The study reports a first comprehensive approach to the proteome analysis done by comparing the protein profiles characteristic of bee- and manually-collected common dandelion (Taraxacum officinale) pollen. Proteins isolated from the bee- and manually-collected pollen was fractionated by two-dimensional electrophoresis, visualized by staining with Colloidal Coomassie G-250, and after tryptic-digestion analysed by mass spectrometry. A computer-assisted analysis was performed for comparison of protein profile maps typical of bee- and manually-collected dandelion pollen.

Keywords:

• Common dandelion
• pollen
• proteome
• protein fold change

Acknowledgements

This research was funded by a grant (No. SVE-01/2012) from the Research Council of Lithuania. The authors thank dr. Mindaugas Valius, dr. Algirdas Kaupinis, dr. Marija Ger and Ilona Zaikova for the excellent assistance with mass spectrometry. The research was partly supported by the long-term research programme “Biopotential and quality of plants for multifunctional use” implemented by the Lithuanian Research Centre for Agriculture and Forestry.

Disclosure statement

The authors declare no conflict of interest.