Antioxidant activity and phenolic composition of propolis from Marmara region, Turkey

Abstract

This study investigated various properties of raw propolis samples from Marmara region of Turkey. Total phenolic content (TPF), total flavonoid (TFC) values, condensed tannin content (CTC) and phenolic profiles of ethanolic extracts (70%) were analyzed. Antioxidant activities were tested using the Ferric (III) reduction/antioxidant capacity (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging methods. Phenolic profiles of the samples were analyzed using high performance liquid chromatography (RP-HPLC-UV) method. TPC, TFC and CTC values in the samples ranged from 52.9 to 203.5 mg GAE/g, 13.6 to 43.9 mg QUE/g, and 2.0 and 2.9 mg CTE/g, respectively. Caffeic acid phenyl ester (CAPE), chrysin, pinocembrin, rutin, and t-cinnamic acid were detected as the common phenolic components in all samples. In conclusion, Marmara region propolis is a rich source of bioactive compounds, especially flavonoids with antioxidant properties.

Keywords:

• Propolis
• phenol
• flavonoid
• antioxidant
• Marmara region

Acknowledgements

We thank to Assoc.Prof.Dr. Meral Kekecoglu for some propolis samples and Assoc.Prof.Dr. Aycan Tosunoglu for their botanical origins.

Disclosure statement

The authors state no potential conflict of interest to be reported.

Additional information

Funding

This work was supported by the Bursa Uludag University Research Unit for providing financial assistance (BAP-AGAM project No. 2016/2).