Occurrence and distribution of Nosema ceranae in honey bee colonies in the Comoros Islands

Abstract

Nosemosis is a parasitic disease caused by microsporidian pathogens of the genus Nosema infecting both the Western honey bee, Apis mellifera, and the Asian honey bee Apis cerana. The disease may adversely affect bee colonies and eventually result in high losses in apiculture and agriculture. We determined the Nosema species infecting honey bees and their prevalence in two islands of the Comoros Archipelago (Grande Comore (GCO) and Mohéli (MOH)) in the Southwest Indian Ocean. In Comoros, beekeeping is largely traditional and practiced at subsistence-level, including honey hunting. Five honey bee foragers from 69 colonies in different localities within the two islands were selected and screened for Nosema infection using microscopy and Polymerase Chain Reaction-restriction fragment length polymorphism (PCR-RFLP). Selected positive samples were sequenced to confirm the species identified using PCR-RFLP. We also examined spore loads to determine the intensity of Nosema infections. Nosema ceranae is the only species detected in the two Comoros islands. High prevalence occurred in the large island, GCO (73.9%), while MOH (25.0%) had a low prevalence. Spore counts indicated higher infection intensities in GCO compared to MOH. Generally, PCR-RFLP underestimated the prevalence, although microscopic and molecular diagnostics were well in agreement at the colony level. MOH had lower prevalence and infection intensity compared to GCO, which is the larger island with a higher density of human population. We postulate that the lower levels of pathogen prevalence in MOH in comparison to GCO could arise from minimal human interference.

Keywords:

• PCR-RFLP
• microsporidia
• Apis mellifera
• spore count
• molecular diagnostics

Acknowledgments

We thank J. W. Kilonzo and A. M. Nobataine for their support during field collections. We also thank Dennis A. Wakhungu for support during lab work. The authors gratefully acknowledge the financial support for this research by the following organizations and agencies: International Fund for Agricultural Development (IFAD), Grant number 2000000282; the Swedish International Development Cooperation Agency (Sida); the Swiss Agency for Development and Cooperation (SDC); the Australian Centre for International Agricultural Research (ACIAR); the Federal Democratic Republic of Ethiopia; and the Government of the Republic of Kenya. The views expressed herein do not necessarily reflect the official opinion of the donors. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Disclosure statement

The authors report there are no competing interests to declare.

Data availability statement

The data that support the findings of this study are available on request from the corresponding author [HMGL]. DNA sequences were deposited at GenBank under the accession numbers: OP805630-OP805648.