ENZVU —an enzyme kinetics computer simulation based upon a conceptual model of enzyme action

Abstract

The value of simulation and gaming in biology is briefly reviewed and the principles applied to enzyme kinetics in the form of a conceptual model of enzyme action based upon the ability of computers to generate random numbers. Enzyme movement and catalysis are controlled by the generation of appropriate random x, y-coordinates which determine the location of molecules within a two-dimensional reaction grid. The data generated by the simulation are subjected to kinetic analysis with great emphasis placed upon the value of the direct linear plot for K_m and V_max determination. The advantage of this form of kinetic analysis for data containing errors compared with the traditional kinetic plots is discussed. The simulation is appropriate for school use when used in a simplified form and, with more rigorous treatment, higher education. The random number generator can be utilized to produce realistic data containing variable error and the general principles involved are of widespread application to simulations for many biological phenomena where for example, success or failure, positional change, or time-related events are involved.