New insights on the external features of egg capsules and embryo development in the squid Loligo vulgaris

Abstract

The embryonic development of the squid Loligo vulgaris was observed from 183 egg masses collected from special devices deployed throughout Cabrera National Park (Baleares Islands, western Mediterranean Sea). Sequence alignment analysis of the cytochrome oxidase I gene revealed that all embryos belonged to L. vulgaris. In total, 549 egg capsules were examined. Viable egg capsules (n = 420) were classified into one of five maturation stages according to the primary external features. The length of the viable egg capsules varied between 40 and 170 mm, and increased with embryonic development. The non-viable capsules (n = 129) were categorized into four groups: I (Ginger root), non-viable II and III, and empty egg capsule (IV). The percentage of non-viable capsules (i.e. grades I, II and III) was 92.25%. Empty capsules accounted for 7.75% of the total non-viable egg capsules. Embryonic development was classified into a second scale of eight stages. Egg capsule stage and embryonic stage were significantly related (n = 420; p < 0.001), facilitating the determination of the embryo developmental phase based on the outward appearance of the egg capsules. The embryo development stage based on the external features of the egg capsules might constitute an innovative tool for in situ embryological data collection. This new method is neither time consuming nor invasive, and could be helpful in fishing cruises, for scuba diving visual census in natural habitats and for laboratory culture. Slight variability in the developmental embryonic stages within egg capsules from the same egg mass was identified. The origin of this asynchrony is discussed. Chronological appearance of organs was similar to that of the six loliginid species previously examined. However, some developmental changes in the timing or rate of events (heterochronies) were observed: Hoyle’s organ was formed earlier in L. vulgaris and the appearance of ventral chromatophores was slightly delayed (2 days) compared with the other species considered.

Keywords:

• embryonic development
• heterochronies
• egg capsules
• Loligo vulgaris

Acknowledgements

The authors would like to thank friends, biologists and the keepers of Cabrera National Park for invaluable assistance in the development of various aspects of this study, particularly Cayetana Casas (IMEDEA), Mercè Morató (IEO-COB), Carolina Vidal (Palma Aquarium-IMEDEA) and Paco Valdés (SIETEMARES), for participation in the diving operations. The authors would also like to thank two anonymous referees for constructive comments.

Additional information

Funding

This work was financially supported through the Organismo Autónomo de Parques Naturales de España of the Ministerio de Agricultura, Alimentación y Medio Ambiente (CEFAPARQUES, Project number: 458/2011).