Community-level plant–pollinator interactions in a Palaeotropical montane evergreen oak forest ecosystem

ABSTRACT

The montane terrain of northern Laos is covered by species-rich subtropical evergreen oak forests, home to endemic tree genera such as Mytilaria (Hamamelidaceae), and characterised by the coexistence of several honeybee and bumblebee species. We explored community-level plant–pollinator interactions of this unique little-known ecosystem. Extensive direct observations on flowering phenology and flower-visitor assemblages of 288 plant species of 82 families were conducted in a montane forest ecosystem in Houaphanh and Xiangkhouang Provinces, Laos, from 2005 until 2016. Based mainly on the extensive flower-visit data, we assessed the pollination system of each plant species. Five sympatric honeybee species (Apis dorsata, A. laboriosa, A. cerana, A. florea and A. andreniformis) were common on various types of flowers, and floral preferences differed among species. Long-tongued bees belonging to Bombini and Anthophorini (Apidae) were species-rich and frequent visitors on various deep flowers, especially Acanthaceae, Balsaminaceae, Lamiaceae, Rubiaceae and Zingiberaceae. Character displacement by tongue length was observed among the bee species, and many relaxed species-specific and species-semispecific interactions were observed between the bees and the deep-flowers. Four plant species, in the genera Mytilaria, Chloranthus, Dioscorea and Cryptocarya, were visited exclusively by thrips. Two plant species, in the genera Lysimachia and Thladiantha, had oil-secreting flowers, which were specifically visited by the oil-collecting bees Macropis and Ctenoplectra, respectively. The dominant pollination system assessed was general insect pollination (31%), followed by long-tongued bee, small bee, honeybee, dipteran, lepidopteran, beetle, wasp, carpenter bee, thrips (e.g. the endemic genus Mytilaria), bird and hemipteran pollination. Our results suggest that the plant–pollinator interactions in the Palaeotropic montane ecosystem are characterised by significant contribution of the five honeybee species and species-rich, morphologically diverse long-tongued bees, both of which have contributed to shaping the remarkable diversity of angiosperms with deep flowers.

KEYWORDS:

• Amegilla
• Apis
• bumblebee
• Castanopsis
• Impatiens
• long-tongued bee
• pollination system

Acknowledgements

The authors thank H. Chanthavong, L. Xayvongsa, A. Vilayphone, D. Thongphan, K. Phomphoumy, N. Mahindhorathep, V. Phimpasone, L. Siakor, V. Sihapanya, B. Khomvixam, K. Khamphilavong and other staff of the National University of Laos, and the Provincial Agriculture and Forestry Office of Houaphan Province and Xiengkhuang Province for their assistance and permission to study in Laos.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

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Additional information

Funding

This work was supported by a Japan Ministry of Education, Culture, Science, Sports, and Technology Grant-in-Aid for Scientific Research (#15370012, #18207002, #22247003, #22405009, #15H02420) and by Research Institute for Humanity and Nature (#4-2).