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Summary
Five enzymes in the crude digestive gland extracts of individual snails of the B. tropicus/truncatus complex have been analysed by isoelectric focusing in polyacrylamide gels. The enzymes studied were: malate dehydrogenase (MDH); phosphoglucomutase (PGM); glucosephosphate isomerase (GPI); acid phosphatase (AcP) and hydroxybutyrate dehydrogenase (HBDH). Snails from 103 population samples (52 diploid, 51 tetraploid) from diverse geographical origins have been studied. Diploid and tetraploid snails were clearly differentiated and regional distributions of certain enzyme types are reported. The taxonomic implications of the findings are discussed.