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ABSTRACT
Sixteen predominantly male Mendelian mutants were isolated. All belong to one of the four linkage groups of predominantly male mutants previously described (mal-1, mal-2, mal- 3 and mal-4). Predominantly female mutants if-1 and f-3) segregating in a Mendelian fashion were also isolated. The male mutants mal-2, mal-3 and mal-4 are epistatic to f-1 and f-3, as double mutants (f mal) are predominantly male. In contrast, f-1 mal-1 double mutants are predominantly female, while f-3 mal-1 double mutants are wild type-like, or have an excess of male gametangia. Strains with f-1 and f-3 develop up to 50% male gametangia when the incubation temperature is lowered from the normal 35 C to 23 C. Suppressor mutations (s— phenotypically wild type-like) of mal-1 were also found, and s mal-1 double mutants are hermaphroditic as is the wild type.