ABSTRACT
The nutritional requirements of Acremonium coenophialum, a fungus endophyte oftall fescue, were examined in a semi-defined liquid medium. The fungus utilized several carbon sources including fructose, glucose, mannose, sucrose, trehalose, raffinose, sorbitol and mannitol. Trehalose and mannitol were excellent carbon sources. Nitrogen sources utilized by the endophyte included ammonium, arginine, asparagine, cysteine, glutamine, proline and serine. Undefined complex nitrogen sources such as yeast extract, soytone and tryptone supported excellent growth. In a defined medium the fungus exhibited a requirement for thiamine.
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