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Abstract
Techniques of freeze-fracturing and cytoplasmic maceration were combined to reveal the septal pore cap in some members of the Rhizoctonia s.l. complex by the use of field emission gun-scanning electron microscopy. Perforate septal pore caps were observed in Rhizoctonia solani, Aquathanatephorus pendulus, Ceratobasidium cornigerum, and Waitea circinata, showing a successive decrease in cap size. W. circinata revealed an irregular distribution of holes in the cap. Imperforate septal pore caps were observed in Epulorhiza anaticula and Sebacina vermifera. The cap of E. anaticula was connected to tubular endoplasmic reticulum, while the cap of S. vermifera was connected to platelike endoplasmic reticulum. Scanning microscopy is a useful method to complement our knowledge of the septal pore cap as it shows the septal pore cap in situ in relation with other organelles. Septal pore caps present within the Rhizoctonia s.l. complex can be subdivided into: (i) a perforate septal pore cap with either regularly or irregularly distributed holes; (ii) an imperforate septal pore cap connected with tubular endoplasmic reticulum or with platelike endoplasmic reticulum. Our results sustain the current classification of the investigated species in four orders.