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Analysis of morphological and ecological characters plus ribosomal DNA sequences support the removal of seven related tropical species from the genus Tricholoma. This group of apparently nonmycorrhizal species that have abundant clamp connections on all hyphae but lack siderophilous granulation in the basidia cannot be accommodated in other genera, such as Calocybe or Megatricholoma. We therefore propose Macrocybe as a new genus in the Tricholomataceae for these taxa.
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