ABSTRACT
Species of Pulveroboletus (Boletaceae, Boletales) in China are investigated on the basis of morphology and molecular phylogenetic analyses of DNA sequences from nuc rDNA region encompassing the internal transcribed spacers 1 and 2, along with the 5.8S rDNA (ITS) and nuc 28S rDNA D1–D2 domains (28S) and sequences from the translation elongation factor 1-α gene (TEF1). Nine species are recognized in the country, three of them are described as new: P. flaviscabrosus, P. rubroscabrosus, and P. subrufus, five of them are previous described taxa: P. brunneopunctatus, P. brunneoscabrosus, P. macrosporus, P. reticulopileus, and P. sinensis, the remaining one is tentatively named P. cf. ridleyi, which will be further studied in the future. A key to the Chinese taxa of the genus is provided. Unexpectedly, the current study did not identify disjunct populations of Pulveroboletus species in East Asia and North/Central America.
ACKNOWLEDGMENTS
The authors thank the following colleagues: Dr. E.D. Liu, Dr. X.Y. Wang, and Dr. W.Z. Ma (Kunming Institute of Botany [KIB], Chinese Academy of Sciences [CAS]) and Dr. B. Ortiz-Santana (US Forest Service, Northern Research Station, Center for Forest Mycology Research) for providing some specimens of Pulveroboletus on loan; Dr. Q. Cai (KIB, CAS) and Mr. M.S. Su (Jiangxi University of Traditional Chinese Medicine) for providing important collections of Pulveroboletus; and Mr. O. Raspé (Botanic Garden Meise, Nieuwelaan) for providing TEF1 sequence of Pulveroboletus fragrans. The authors also thank the anonymous reviewers for their valuable suggestions and comments to the improvement of our work.
FUNDING
The study was supported by the National Natural Science Foundation of China (nos. 31210103919, 31360008, 31560005) and the Key Laboratory for Plant Diversity and Biogeography of East Asia, KIB, CAS (KLBB 201303).