ABSTRACT
Some Amanita specimens collected from Malaysia are critically investigated by morphological examination and molecular analysis of two gene fragments, the nuc rDNA partial 28S (28S) gene and the internal transcriber spacer (ITS1-5.8S-ITS2 = ITS) regions. Six phylogenetic species of Amanita section Caesareae are recognized among the studied collections. One of them is described as new, A. malayensis. Four of the phylogenetic species correspond with existing morphology-based taxa: A. aporema, A. javanica, A. princeps, and A. similis. The remaining species is not described because of the paucity of material. Detailed descriptions and the distribution of these southeastern Asian species are provided, along with a key to the species of section Caesareae from Malaysia.
ACKNOWLEDGMENTS
The authors are very grateful to the curators of Forest Research Institute Malaysia (FRIM); Herbarium of Cryptogams, Kunming Institute of Botany of the Chinese Academy of Sciences (HKAS); Herbarium of Institute of Mycological Sciences and Technology, Ludong University (HMLD); Mycological Herbarium of Hunan Normal University (MHHNU); and Forestry and Forest Products Research Institute, Tsukuba (TFM). Two anonymous reviewers are also gratefully acknowledged for their detailed comments and constructive suggestions. The authors are very appreciative of Prof. Philip S. Ward (University of California, Davis, USA) for kindly checking the English in the revision of this paper.
FUNDING
This work was supported financially by the National Natural Science Foundation of China and Yunnan Provincial Government (nos. U1302263 and 31560004), Yunnan Applied Basic Research Projects–Joint Special Project (no. 2014FB016), the Science Research Foundation of Department of Education, Yunnan Province (no. 2015Y147), and the Open Research Foundation of Yunnan Key Laboratory of Pharmacology for Natural Products (no. 2015G003).
Supplemental data
Supplemental data for this article can be accessed on the publisher’s Website.