Novel taxa in Cladochytriales (Chytridiomycota): Karlingiella (gen. nov.) and Nowakowskiella crenulata (sp. nov.)

ABSTRACT

Six Nowakowskiella species from Brazil were identified and purified on corn meal agar (CMA) plus glucose and Peptonized Milk-Tryptone-Glucose (PmTG) media and placed into a phylogenetic framework for the genus. New sequence data are presented for four species: N. elongata, N. multispora, and N. ramosa and the new species N. crenulata. Our maximum likelihood and Bayesian analyses of combined 18S, 5.8S, and 28S subunits of nuc rDNA showed that Nowakowskiella is not a monophyletic clade because of the position of Nowakowskiella elongata, which is more related to Cladochytrium. We reclassify N. elongata as the type of a new genus, Karlingiella.

KEYWORDS:

• Chytrid
• phylogeny
• 3 new taxa

ACKNOWLEDGMENTS

Lucas Michelotti, Denise C. Bicudo, Samantha B. Faustino, Elaine C.R. Bartozek, Stefano Zorzal de Almeida, and Maria A. P. C. da Silva (Dorinha) are recognized for their contribution collecting samples or laboratory support, and we thank Instituto Florestal and Grupo Votorantim for the permission to collect samples.

Additional information

Funding

This study was supported by Fapesp (Fundação de Amparo a Pesquisa do Estado de São Paulo) through scholarships awarded to G. H. Jerônimo (nos. 2014/16358-4 and 2016/25800-6; BEPE) and A. L. Jesus (no. 2016/00697-0) and projects to C. L. A. Pires-Zottarelli (nos. 2012/50222-7 and 2016/11146-4). We are indebted to CAPES (Coordenação de Aperfoiçoamento de Pessoal de Nível Superior), which supported G. H. Jerônimo during his PhD research. Work in the laboratory of T.Y.J. was funded by NSF grant DEB-1354625. Additionally, we thank the thematic Acquased project (FAPESP no. 2009/53898-9) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the productivity fellowship awarded to C. L. A. Pires Zottarelli (no. 304493/2015-5).