Systematic analysis of Russula in the North American Rocky Mountain alpine zone

ABSTRACT

Russula (Russulales) is an important ectomycorrhizal fungal genus in Arctic and alpine regions where it occurs with Salix, Betula, Dryas, and Polygonum, yet a complex phylogenetic analysis of the genus in these habitats is lacking. This research compared collections of Russula from the Rocky Mountain alpine (Colorado, Montana, Wyoming) with reference specimens from Arctic and alpine habitats, mostly in Europe, using an in-depth morphological study and a phylogenetic analysis of the nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS barcode) and the second largest subunit of the RNA polymerase II gene (rpb2). One hundred thirty-nine Russula collections were sequenced, including type material. Ten species are reported from alpine or treeline habitats in the Rocky Mountains. This is the first formal report of R. cf. altaica, R. saliceticola, and R. subrubens from the Rocky Mountains and of R. purpureofusca in North America. Russula laevis is reported for the first time under this name with a voucher, and not as an environmental sample. Previous reports of R. nana and R. laccata are molecularly confirmed. Two species are reported from subalpine habitats at treeline: R. montana with conifers and R. cf. altaica with Betula. In this study, R. laccata, R. subrubens, and R. laevis were collected in alpine habitats but have been reported below treeline in Europe; these species may also be present at lower elevations in North America. Most species have an intercontinental distribution and have been reported in other alpine or Arctic habitats. Two unidentified and potentially new species were only found in North America and are discussed. A key to the alpine Russulas of North America is provided.

KEYWORDS:

• Betula
• Dryas
• ITS
• rpb2
• Russulaceae
• Salix
• taxonomy

ACKNOWLEDGMENTS

We thank Dr. Egon Horak, Jukka Vauras, and Vera Evenson and all herbaria that provided loans of material, Matt Lavin for training in phylogenetic analysis, and Sue Brumfield for assisting with the scanning electron microscope (SEM) photographs. We would also like to thank the reviewers who improved the quality of the manuscript.

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Additional information

Funding

We thank the John W. Marr Fund, the Ben Woo Scholarship from Puget Sound Mycological Society, the Southwest Montana Mycological Society, The Department of Plant Sciences and Plant Pathology at Montana State University (MSU), and the College of Agriculture at MSU for funding.