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Abstract
An antigen of Acremonium loliae Latch, Christensen and Samuels can be solubilised from dried ryegrass samples by resuspending ground plant tissue in buffer containing Tween 20 (0.2%) at 5°C. Additional ovalbumin coating increases the antigenic reactivity of samples and decreases replicate variance. This antigen solubilisation procedure is as effective in detecting the A. Loliae antigen as grinding samples with a pestle and mortar, and is less time consuming.