Concentrating Giardia cysts in water by tangential flow filtration compared with centrifugation

Joanna Chaste Massey University/New Zealand Health Department Giardia Unit, Department of Microbiology and Genetics, School of Biological Sciences, Massey University, Private Bag, Palmerston North, New Zealand

Philip J. Kelly Massey University/New Zealand Health Department Giardia Unit, Department of Microbiology and Genetics, School of Biological Sciences, Massey University, Private Bag, Palmerston North, New Zealand

Tim J. Brown Massey University/New Zealand Health Department Giardia Unit, Department of Microbiology and Genetics, School of Biological Sciences, Massey University, Private Bag, Palmerston North, New Zealand

Abstract

Centrifugation, tangential filtration, and sedimentation were compared as methods of concentrating cyst‐containing wash water from filters. A range of cyst concentrations were used on duplicate samples. The cysts were concentrated by one of the above methods and the detectable number of cysts noted. The ability of the methods to process samples with sediment and the overall time taken to process a sample was assessed. The method of choice for the concentration of Giardia cysts in water is centrifugation.

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Concentrating Giardia cysts in water by tangential flow filtration compared with centrifugation

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Concentrating Giardia cysts in water by tangential flow filtration compared with centrifugation

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