Characterisation of the expression of select genes in response to key marine pollutants in the triplefin Forsterygion capito

ABSTRACT

Biomarkers in receptor species can be used to assess and monitor ecosystem health. This study aimed to develop mRNA biomarkers in the New Zealand triplefin Forsterygion capito and validate their induction in response to common estuarine contaminants. The targeted genes were cytochrome P450 1A (cyp1a), metallothionein (mt), vitellogenin (vtg), and cytochrome P450 aromatase (cyp19b). Fish were treated with intraperitoneal injections of benzo[a]pyrene (BaP), zinc, and 17β-oestradiol (E₂). Levels of cyp1a were upregulated in the livers of fish exposed to BaP, and the vtg gene was up-regulated in male fish exposed to E₂. These results indicated that these two biomarkers in F. capito can be used to assess exposure to contaminants exerting similar mechanisms of toxicity.

KEYWORDS:

• Biomarker
• quantitative PCR
• New Zealand native fish
• benzo[a]pyrene
• 17β-oestradiol

Acknowledgements

DM thanks Emily Frost.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

DM was funded by a BECAS-CHILE, CONICYT scholarship.