Abstract
Aims: Comparison between BRCA1‐associated and sporadic ovarian carcinomas is a potential method to identify candidate modifier gene/s involved in the carcinogenic pathway of either or both groups. A previous study identified a significant difference in the frequency of copy number gain at 2q24–q32 by comparing BRCA1‐associated and sporadic ovarian tumour specimens using comparative genomic hybridisation (CGH). The present study aimed to investigate the reported allelic imbalance at 2q24–32 by amplification of several microsatellite markers at the region by quantitative microsatellite analysis (QuMA) using Taqman at the same region identified as a site of allelic imbalance.
Methods: The copy number of the genomic region in 2q24–32 was established in 21 BRCA1‐associated ovarian carcinomas and 14 sporadic cases using quantitative microsatellite polymerase chain reaction (PCR). Statistical analysis was performed using permutation test analysis.
Results: A significant loss at D2S156 marker (2q24.2) (p = 0.026) compared with the other three markers at 2q24–32 was found in the sporadic cohort but not in the BRCA1‐associated group (p = 0.385).
Conclusions: Our data do not support the association between copy number gain at 2q24–32 and BRCA1 mutation status in ovarian cancers reported previously. The novel finding of the present study was significant loss at 2q24.2 in sporadic ovarian cancers.