Phylogeny and genetic diversity of the Philippine eucheumatoid genus Mimica (Solieriaceae, Rhodophyta), and the proposal for Kappaphycopsis gen. nov. to include the anomalous species of Kappaphycus, K. cottonii

ABSTRACT

The term ‘eucheumatoids’ designates a group of genera in the family Solieriaceae that are the source of some of the most valuable algal products in cultivation. While economically important in the tropics, much of its taxonomy is yet to be fully resolved. The eucheumatoid species Mimica arnoldii is a Batesian mimic of corals that was recently transferred to its own genus from Eucheuma based primarily on peculiarities of habit and a lack of medullary rhizoids, in contrast to the generitype Eucheuma denticulatum that has the latter. We studied the diversity of Mimica arnoldii from samples collected in the Philippines to determine their genetic variation, relationships to other eucheumatoids, and whether its two distinct morphological expressions, which have been named as varieties, are genetically different. We produced a phylogeny using a three-gene concatenated dataset, and used the variable mitochondrial cox2-3 spacer to determine intraspecific variation and the degree of correlation between sequence-variation and varietal status. Our results showed that Mimica is a distinct genus, and that intra-specific molecular data did not correspond to the morphology-based varieties, although it did correlate with geographical distribution. Our results also indicate that Kappaphycus, as presently circumscribed, contains species with (Kappahycus alvarezii, K. striatus and K. malesianus) and without (Kapppahycus cottonii) internal medullary hyphal core, and that K. cottonii is sister to the remaining Kappaphycus species. We thus propose Kappaphycopsis gen. nov. for K. cottonii for the species of Kappaphycus without internal medullary rhizoids (anaxiferous).

KEYWORDS:

• Anaxiferous eucheumatoids
• COI-5P
• cox2-3
• Eucheuma
• Mimica arnoldii
• Molecular-assisted alpha taxonomy
• Morphotype
• rbcL

ACKNOWLEDGEMENTS

C.R.G. Lorenzo, J.F. Agluba, C.D.Q. Rivadelo, J.R.DL. Estipular, J.J.R. De Guzman, and D.L.P. Lagatuz contributed additional photo and sequence dataset. Gerald T. Kraft provided sage advice for improving the manuscript. John McNeil helped with nomenclature. Additional comments were provided by A.J. Calado. This work was supported in part through a grant to R.V. Dumilag from the Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) Science Education Institute through an Accelerated Science and Technology Human Resources Development Programme (DOST-SEI ASTHRDP), the Office of the Research, Development and Extension Services (ORDES) of the Sorsogon State University and via strategic research funds from the School of Biological Sciences at Victoria University of Wellington to G.C. Zucccarello.

DISCLOSURE STATEMENT

No potential conflict of interest was reported by the authors.

SUPPLEMENTARY MATERIAL

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