Isolation of Human Clq as a by-Product of Routine Fractionation

C. Granier Department of Fractionation, Centre National de Transfusion Sanguine, Avenue des Tropiques, 91400, Orsay-Courtaboeuf, France

A. Faure Department of Fractionation, Centre National de Transfusion Sanguine, Avenue des Tropiques, 91400, Orsay-Courtaboeuf, France

R. Tavernier Department of Fractionation, Centre National de Transfusion Sanguine, Avenue des Tropiques, 91400, Orsay-Courtaboeuf, France

M. Steinbuch Department of Fractionation, Centre National de Transfusion Sanguine, Avenue des Tropiques, 91400, Orsay-Courtaboeuf, France

Abstract

Clq can be isolated as a by-product of large scale fractionation. The euglobulins of fraction III are submitted to chromatography on CM-cellulose in a batch-procedure. The active fraction being frequently contaminated by lysozyme the protein can be further purified by gel-filtration in the presence of carbohydrates. The purified Clq is suitable for the detection of immune complexes.

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Isolation of Human Clq as a by-Product of Routine Fractionation

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Isolation of Human Clq as a by-Product of Routine Fractionation

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