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Original Article

Amino acid synthetic media for fungal pathogens based on aminopeptidase specificities: Histoplasma capsulatum, Blastomyces dermititidis, Paracoccidioides brasiliensis and Cryptococcus neoformans.

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Pages 142-147 | Published online: 09 Jul 2009
 

Abstract

The development of simple and chemically defined liquid media for Histoplasma capsulatum, Blastomyces dermatitidis, Paracoccidioides brasiliensis and Cryptococcus neoformans according to their aminopeptidases profiles as amino acid requirement was described. When 1·5% purified agar was added, these media also supported excellent mycelial growth and sporulation of the deep mycoses. H. capsulatum was converted to and maintained in yeast phase when 0·1% L-cystine was added to the solid medium incubated at 37°C.

Zusammenfassung

Die Entwiklung eines einfachen und chemisch definierten flüssigen Nährbodens für Histoplasma capsulatum, Blastomyces dermatitidis, Paracoccidioides brasiliensis, und Cryptococcus neoformans wird beschrieben, das dem Aminosäurebedürfnis entspricht, wie es auf Grund ihrer Aminopeptidasen—Leistungsprofile bestimmt wurde. Wenn wir 1,5% gereinigten Agar zugaben, ermöglichten diese Nährböden auch ausgezeichnetes Mycelwachstum und Sporulation der tiefen Mykosen. Das H. capsulatum konnte in die Hefephase überführt und erhalten werden, wenn 0·1% L-Cystin dem Nährboden (bei 37°C bebrütet) zugefügt wurde.

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