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Original Article

Coccidioides immitis: Su aislamiento de muestras de suelos de las provincias de San Luis y Mendoza

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Pages 51-57 | Published online: 09 Jul 2009
 

Summary

By using the double pour plate method, Yeasts Extract Agar (YE) which contained a high concentration of chloramphenicol-streptomycin sulfate and cycloheximide, and by inoculating mice intraperitoneally, we succeed in the isolation of different strains of Coccidicides immitis from 12 samples of the soils collected in the province of San Luis and from 1 of the province of Mendoza (Argentina), a 70% of the samples turned out positive.

Identification of the strains was based on macro- and micro-morphology of the colonies, pathogenicity for mice and the capacity to transform in shaked cultures.

Abstract

Mediante la técnica de siembra en doble capa y utilizando el medio de agar extracto de levaduras, adicionado de cicloheximida en alta concentración, con cloramfenicol-estreptomicina y la inoculación intraperitoneal en ratones, se aislaron, distintas cepas del hongo Coccidicides immits en 12 muestras de tierra de la provincia de San Luis y 1 de la provincia de Mendoza (en el 70% de los lugares investigados).

Se indentificaron posteriormente por su macro y micromorfología, su patogenicidad en ratones y su capacidad de transformación morfológica en cultivo agitado.

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