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Original Article

Histoplasma capsulatum: Chemical variability of the yeast cell wall

, &
Pages 279-284 | Published online: 09 Jul 2009
 

Abstract

Four strains of Histoplasma capsulatum were analyzed to observe any variations in their cell wall composition. Strain G-184B produced the same amount of sugars reported by Domer (2) although qualitative analyses agreed with Kanetsuna's report (4) as far as the presence of glucose, galactose, and mannose was concerned. Our results agree with previous reports in classifying H. capsulatum into chemotypes I and II, the latter having more α-glucan in its cell wall. A decrease in the amount of fraction 2 (α-glucan) of strain G-184B was observed when the strain was subcultured in vitro for many years, an effect similar to that reported for Paracoccidioides brasiliensis (12, 13).

Resumen

Se analizaron cuatro cepas de Histoplasma capsulatum a fin de observar posibles variaciones en la composición de sus paredes celulares. La cepa G-184B produjo la misma cantidad de azúcares reportados por Domer (2) aunque los análisis cualitativos estuvieron de acuerdo con el trabajo de Kanetsuna y col. (4) quienes reportaron glucosa, galactosa y manosa como integrantes de la pared celular. Nuestros resultados están de acuerdo con trabajos anteriores que dividen H. capsulatum en quimiotipos I y II, el último de los cuales posee α-glucán en su pared celular. Se observó una disminución en la cantidad de fracción 2 (α-glucán) en la cepa G-184B al subcultivar la cepa in vitro por varios años, efecto similar al reportado anteriormente en Paracoccidioides brasiliensis (12, 13).

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